IDBA_HYBRID(1)

NAME
idba_hybrid - Iterative de Bruijn Graph Assembler for hybrid sequencing data

SYNOPSIS
idba_hybrid -r read.fa -o output_dir [--reference ref.fa]

DESCRIPTION
IDBA-Hybrid - Iterative de Bruijn Graph Assembler for hybrid sequencing data.

  Allowed Options:

-o, --out arg (=out)
    output directory

-r, --read arg
    fasta read file (<=128)

--read_level_2 arg
    paired-end reads fasta for second level scaffolds

--read_level_3 arg
    paired-end reads fasta for third level scaffolds

--read_level_4 arg
    paired-end reads fasta for fourth level scaffolds

--read_level_5 arg
    paired-end reads fasta for fifth level scaffolds

-l, --long_read arg
    fasta long read file (>128)

--reference arg
    reference genome

--mink arg (=20)
    minimum k value (<=124)

--maxk arg (=100)
    maximum k value (<=124)

--step arg (=20)
    increment of k-mer of each iteration

--inner_mink arg (=10)
    inner minimum k value

--inner_step arg (=5)
    inner increment of k-mer

--prefix arg (=3)
    prefix length used to build sub k-mer table

--min_count arg (=2)
    minimum multiplicity for filtering k-mer when building the graph

--min_support arg (=1)
    minimum supoort in each iteration

--num_threads arg (=0)
    number of threads

--seed_kmer arg (=30)
    seed kmer size for alignment

--min_contig arg (=200)
    minimum size of contig

--min_region arg (=500)
    minimum size of region in reference genome

--similar arg (=0.95)
    similarity for alignment

--max_mismatch arg (=3)
    max mismatch of error correction

--min_pairs arg (=3)
    minimum number of pairs

--max_gap arg (=50)
    maximum gap in reference

--no_local
    do not use local assembly

--no_coverage
    do not iterate on coverage

--no_correct
    do not do correction

--pre_correction
    perform pre-correction before assembly